Dissecting the Molecular Anatomy of Tissue / edited by Michael R. Emmert-Buck, John W. Gillespie, Rodrigo F. Chuaqui.

Springer eBooks

Clinkal Issues and Tissue Processing -- Molecular Pathology Laboratory of the Future -- Clinical Utilities of Microdissected Samples -- Tissue Processing -- Sample Management and Tracking -- Methods of Tissue Microanalysis -- Tissue Microarrays -- Molecular Analysis of Microdissected Samples -- Methods of Microdissection and New Technologies -- Standard Diagnostic Techniques -- DNA Applications -- mRNA Applications -- Proteomic Applications -- Protocols for Tissue Handling and Molecular Analysis.

The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.

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