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020 _a9780387764979
_99780387764979
024 7 _a10.1007/9780387764979
_2doi
035 _avtls000332684
039 9 _a201509030235
_bVLOAD
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040 _aMX-SnUAN
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_cMX-SnUAN
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050 4 _aQH505
100 1 _aHinterdorfer, Peter.
_eeditor.
_9303576
245 1 0 _aHandbook of Single-Molecule Biophysics /
_cedited by Peter Hinterdorfer, Antoine Oijen.
264 1 _aNew York, NY :
_bSpringer US,
_c2009.
300 _axxiv, 648 páginas 98 ilustraciones, 49 ilustraciones en color.
_brecurso en línea.
336 _atexto
_btxt
_2rdacontent
337 _acomputadora
_bc
_2rdamedia
338 _arecurso en línea
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
_2rda
500 _aSpringer eBooks
505 0 _aSingle-Molecule Fluorescent Particle Tracking -- Single-Molecule Analysis of Biomembranes -- Single-Molecule Imaging in Live Cells -- Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy -- Single-Molecule FRET: Methods and Biological Applications -- Single-Molecule Enzymology -- Single-Molecule Studies of Rotary Molecular Motors -- Fluorescence Correlation Spectroscopy in Living Cells -- Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy -- Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores -- Nanopores: Generation, Engineering, and Single-Molecule Applications -- Single-Molecule Manipulation Using Optical Traps -- Magnetic Tweezers for Single-Molecule Experiments -- Folding of Proteins under Mechanical Force -- Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy -- Probing Single Membrane Proteins by Atomic Force Microscopy -- High-Speed Atomic Force Microscopy -- Recognition Imaging Using Atomic Force Microscopy -- Atomic Force Microscopy of Protein–Protein Interactions -- A New Approach to Analysis of Single-Molecule Force Measurements -- Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation.
520 _aThe last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes: Experimental techniques to monitor and manipulate individual biomolecules The use of single-molecule techniques in super-resolution and functional imaging Single-molecule studies of physical properties of biomolecules Single-molecule enzymology and biochemistry Single molecules in the membrane Single-molecule techniques in living cells Integration of single-molecule biophysics and nanoscience
590 _aPara consulta fuera de la UANL se requiere clave de acceso remoto.
700 1 _aOijen, Antoine.
_eeditor.
_9303577
710 2 _aSpringerLink (Servicio en línea)
_9299170
776 0 8 _iEdición impresa:
_z9780387764962
856 4 0 _uhttp://remoto.dgb.uanl.mx/login?url=http://dx.doi.org/10.1007/978-0-387-76497-9
_zConectar a Springer E-Books (Para consulta externa se requiere previa autentificación en Biblioteca Digital UANL)
942 _c14
999 _c279316
_d279316