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020 _a9781402034664
_99781402034664
024 7 _a10.1007/1402034660
_2doi
035 _avtls000334360
039 9 _a201509030207
_bVLOAD
_c201404120734
_dVLOAD
_c201404090514
_dVLOAD
_y201402041146
_zstaff
040 _aMX-SnUAN
_bspa
_cMX-SnUAN
_erda
050 4 _aQK900-989
100 1 _aGraça, Manuel A.S.
_eeditor.
_9307101
245 1 0 _aMethods to Study Litter Decomposition :
_bA Practical Guide /
_cedited by Manuel A.S. Graça, Felix Bärlocher, Mark O. Gessner.
264 1 _aDordrecht :
_bSpringer Netherlands,
_c2005.
300 _ax, 329 páginas
_brecurso en línea.
336 _atexto
_btxt
_2rdacontent
337 _acomputadora
_bc
_2rdamedia
338 _arecurso en línea
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
_2rda
500 _aSpringer eBooks
505 0 _aLitter Dynamics -- Litter Input -- Leaf Retention -- Manipulation of Stream Retentiveness -- Coarse Benthic Organic Matter -- Leaching -- Leaf Mass Loss Estimated by Litter Bag Technique -- Coarse Particulate Organic Matter Budgets -- Leaf Chemical And Physical Properties -- Determination of Total Nitrogen and Phosphorus in Leaf Litter -- Total Protein -- Free Amino Acids -- Determination of Total Carbohydrates -- Determination of Soluble Carbohydrates -- Total Lipids -- Total Phenolics -- Radial Diffusion Assay for Tannins -- Acid Butanol Assay for Proanthocyanidins (Condensed Tannins) -- Proximate Lignin and Cellulose -- Leaf Toughness -- Microbial Decomposers -- Techniques for Handling Ingoldian Fungi -- Maintenance of Aquatic Hyphomycete Cultures -- An Illustrated Key to the Common Temperate Species of Aquatic Hyphomycetes -- Molecular Approaches to Estimate Fungal Diversity. I. Terminal Restriction Fragment Length Polymorphism (T-RFLP) -- Molecular Approaches to Estimate Fungal Diversity. II. Denaturing Gradient Gel Electrophoresis (DGGE) -- Sporulation by Aquatic Hyphomycetes -- Ergosterol as a Measure of Fungal Biomass -- Acetate Incorporation into Ergosterol to Determine Fungal Growth Rates and Production -- Bacterial Counts and Biomass Determination by Epifluorescence Microscopy -- Secondary Production and Growth of Litter-Associated Bacteria -- Isolation of Cellulose-Degrading Bacteria -- Extraction and Quantification of ATP as a Measure of Microbial Biomass -- Respirometry -- Enzymatic Capabilities -- Extractellular Fungal Hydrolytic Enzyme Activity -- Cellulases -- Viscosimetric Determination of Endocellulase Activity -- Fluorometric Determination of the Activity of ?-Glucosidase and Other Extracellular Hydrolytic Enzymes -- Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase -- Lignin-Degrading Enzymes: Phenoloxidase and Peroxidase -- Phenol Oxidation -- Proteinase Activity: Azocoll and Thin-Layer Enzyme Assay -- Detritivorous Consumers -- Maintenance of Shredders in the Laboratory -- Feeding Preferences -- Data Analysis -- Biodiversity -- A Primer for Statistical Analysis.
520 _aDecomposition of organic matter is a major ecosystem process involving an array of different organisms, including bacteria, fungi and invertebrates. The main objective of this book is to provide students and laboratory instructors at universities and professional ecologists with a broad range of established methods to study plant litter decomposition. Detailed protocols for direct use in the field or laboratory are presented in an easy to follow step-by-step format. A short introduction to each protocol reviews the ecological significance and principles of the technique and points to key references. Although most methods are described for freshwater ecosystems, many will work equally well in the presented or slightly modified form for studies in marine and terrestrial environments.
590 _aPara consulta fuera de la UANL se requiere clave de acceso remoto.
700 1 _aBärlocher, Felix.
_eeditor.
_9307102
700 1 _aGessner, Mark O.
_eeditor.
_9307103
710 2 _aSpringerLink (Servicio en línea)
_9299170
776 0 8 _iEdición impresa:
_z9781402033483
856 4 0 _uhttp://remoto.dgb.uanl.mx/login?url=http://dx.doi.org/10.1007/1-4020-3466-0
_zConectar a Springer E-Books (Para consulta externa se requiere previa autentificación en Biblioteca Digital UANL)
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