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007			cr nn 008mamaa
008			150903s2014 xxu| o |||| 0|eng d
020			_a9781461487944 _99781461487944
024	7		_a10.1007/9781461487944 _2doi
035			_avtls000342590
039		9	_a201509030855 _bVLOAD _c201405050246 _dVLOAD _y201402061130 _zstaff
040			_aMX-SnUAN _bspa _cMX-SnUAN _erda
050		4	_aRC321-580
100	1		_aXiong, Huangui. _eeditor. _9320609
245	1	0	_aCurrent Laboratory Methods in Neuroscience Research / _cedited by Huangui Xiong, Howard E. Gendelman.
264		1	_aNew York, NY : _bSpringer New York : _bImprint: Springer, _c2014.
300			_axiii, 585 páginas 189 ilustraciones, 122 ilustraciones en color. _brecurso en línea.
336			_atexto _btxt _2rdacontent
337			_acomputadora _bc _2rdamedia
338			_arecurso en línea _bcr _2rdacarrier
347			_aarchivo de texto _bPDF _2rda
500			_aSpringer eBooks
505	0		_aSection 1: Neurohistological techniques -- 1. Brain tissue preparation, sectioning and staining -- 2. Brain stereotactic injections -- 3. Tract tracing at light and electron microscopy -- 4. Stereological analysis of neurological tissues -- Section 2: In vitro preparations -- 5. Preparation and use of rodent hippocampal brain slices -- 6.  Single-Cell Neuronal Dissociation For Electrophysiological Studies -- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes -- 8. Isolation and Culture of Neural Stem/progenitor Cells -- 9. Isolation of mitochondria from brain tissue and cells -- 10. Isolation of synaptosomes from archived brain tissues -- 11. Xenopus oocytes isolation and microinjection -- Section 3: Leukocyte isolation and application in neuroscience -- 12. Centrifugal elutriation for studies of neuroimmunity -- 13. Flow cytometry in neuroscience research -- Section 4: Standard Laboratory Nucleic Acid and Protein Detections -- 14. Western Blotting Technique in Biomedical Research -- 15. Polymerase chain reaction (PCR) and Real-time RT PCR -- Section 5: Nanomedicine -- 16. Nanoformulations -- 17. Neuronanomedicine -- Section 6: Bioimaging -- 18. Confocal imaging of nerve cells -- 19. Magnetic resonance imaging (MRI) -- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging -- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography -- 22. Multiphoton Brain Imaging -- Section 7: Neuroelectrophysiology -- 23. Techniques for Extracellular Recordings -- 24. “Blind” patch-clamp recordings from rodent hippocampal slices -- 25. Whole-cell patch clamp recordings -- Section 8: Immunohistochemistry and autoradiography -- 26. Analysis of Receptor Binding And Quantitative Autoradiography -- 27. Techniques in Immunohistochemistry and immunocytochemistry -- Section 9: Analysis of Gene Expression -- 28. Protein identification by mass spectrometry: Proteomics -- 29. DNA Microarrays as a Tool for Neurosciences Research -- 30. Metabolomics -- 31. Bioinformatic Methods and Resources for Neuroscience Research -- Section 10: Animal Models: Behavior and Pathology -- 32. Motor function in rodent models of neurodegenerative disorders -- 33. Humanized mice -- 34. Animal models for PD and ALS -- 35. Animal models for Alzheimer’s Disease -- 36. Animal Models: Behavior and Pathology.
520			_aCurrent Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for  bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
590			_aPara consulta fuera de la UANL se requiere clave de acceso remoto.
700	1		_aGendelman, Howard E. _eeditor. _9304761
710	2		_aSpringerLink (Servicio en línea) _9299170
776	0	8	_iEdición impresa: _z9781461487937
856	4	0	_uhttp://remoto.dgb.uanl.mx/login?url=http://dx.doi.org/10.1007/978-1-4614-8794-4 _zConectar a Springer E-Books (Para consulta externa se requiere previa autentificación en Biblioteca Digital UANL)
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999			_c290078 _d290078