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020 _a9789400745315
_99789400745315
024 7 _a10.1007/9789400745315
_2doi
035 _avtls000367284
039 9 _a201509031045
_bVLOAD
_c201405070439
_dVLOAD
_y201402251615
_zstaff
040 _aMX-SnUAN
_bspa
_cMX-SnUAN
_erda
050 4 _aTP248.6
100 1 _aRenault, Sylvaine.
_eeditor.
_9356432
245 1 0 _aSite-directed insertion of transgenes /
_cedited by Sylvaine Renault, Philippe Duchateau.
264 1 _aDordrecht :
_bSpringer Netherlands :
_bImprint: Springer,
_c2013.
300 _axiii, 380 páginas 54 ilustraciones, 6 ilustraciones en color.
_brecurso en línea.
336 _atexto
_btxt
_2rdacontent
337 _acomputadora
_bc
_2rdamedia
338 _arecurso en línea
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
_2rda
490 0 _aTopics in Current Genetics,
_x1610-2096 ;
_v23
500 _aSpringer eBooks
505 0 _aPreface -- Section 1: Site–directed integration of transgenes -- 1 Transgene site-specific integration : troubles and solutions -- 2 :  Designing non-viral targetd integrating vectors for genome enginnering in vertebrates -- 3: Gene targeting and homologous recombination in Saccharomyces cerevisiae4: Eucaryotic homologous recombination in mammalian cells -- 5 Engineered Zinc Finger Nucleases for Targeted Genome Editing -- 6: Engineered meganucleases for genome engineering purposes -- Section3: Integration based on Site-specific recombination -- 7 : Cre/loxP, Flp/FRT systems and pluripotent stem cell lines -- 8 : Site-specific recombination using C31 Integrase -- 9 : Modified transposases used for site-directed insertion of transgenes -- 10 : Targeted Plasmid Integration into the Human Genome by Engineered Recombinases -- 11: Gene site-specific insertion in plants -- 12 Site-directed insertion of transgenes in Drosophila -- 13. Nuclease Mediated Targeted Genome Modification in Mammalian Cells -- 14: Bio-applications derived from site-directed genome modification technologies.
520 _aThe post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, ?C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.
590 _aPara consulta fuera de la UANL se requiere clave de acceso remoto.
700 1 _aDuchateau, Philippe.
_eeditor.
_9356433
710 2 _aSpringerLink (Servicio en línea)
_9299170
776 0 8 _iEdición impresa:
_z9789400745308
856 4 0 _uhttp://remoto.dgb.uanl.mx/login?url=http://dx.doi.org/10.1007/978-94-007-4531-5
_zConectar a Springer E-Books (Para consulta externa se requiere previa autentificación en Biblioteca Digital UANL)
942 _c14
999 _c313996
_d313996